Evolution of an inhibitory RNA aptamer against T7 RNA polymerase
نویسندگان
چکیده
منابع مشابه
Evolution of an inhibitory RNA aptamer against T7 RNA polymerase
Aptamers are promising gene components that can be used for the construction of synthetic gene circuits. In this study, we isolated an RNA aptamer that specifically inhibits transcription of T7 RNA polymerase (RNAP). The 38-nucleotide aptamer, which was a shortened variant of an initial SELEX isolate, showed moderate inhibitory activity. By stepwise doped-SELEX, we isolated evolved variants wit...
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ژورنال
عنوان ژورنال: FEBS Open Bio
سال: 2012
ISSN: 2211-5463
DOI: 10.1016/j.fob.2012.07.004